Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Acid sphingomyelinase) Polyclonal Antibody, Unconjugated (ER1903-19) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: HepG2(human) cell Lysate at 30 ug MCF-7(human) cell Lysate at 30 ug A431(human) cell Lysate at 30 ug Hale(human) cell Lysate at 30 ug Primary: Anti- Acid sphingomyelinase (ER1903-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64kD Observed band size: 69 kD| Product Name | Acid sphingomyelinase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acid sphingomyelinase ASM ASM_HUMAN aSMase NPD Smpd1 Sphingomyelin phosphodiesterase 1 acid lysosomal Sphingomyelin phosphodiesterase. |
| Product description | Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human Acid sphingomyelinase 201-300/629 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Acid sphingomyelinase ASM ASM_HUMAN aSMase NPD Smpd1 Sphingomyelin phosphodiesterase 1 acid lysosomal Sphingomyelin phosphodiesterase. |
|---|---|
| Molecular Weight(MW) | 64 KDa |
| Cellular Localization | Lysosome. |
| SwissProt ID | P17405 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Acid sphingomyelinase) Polyclonal Antibody, Unconjugated (ER1903-19) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: HepG2(human) cell Lysate at 30 ug MCF-7(human) cell Lysate at 30 ug A431(human) cell Lysate at 30 ug Hale(human) cell Lysate at 30 ug Primary: Anti- Acid sphingomyelinase (ER1903-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64kD Observed band size: 69 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
