Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ack1) Polyclonal Antibody, Unconjugated (ER1903-20) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Spinal cord(Mouse)l Lysate at 40 ug Primary: Anti-Ack1 (ER1903-20) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 119 kD Observed band size:119 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ack1) Polyclonal Antibody, Unconjugated (ER1903-20) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Ack1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acetate kinase 1 Acetokinase 1 Activated CDC42 kinase 1 Activated Cdc42 associated kinase 1 Activated p21cdc42Hs kinase Tyrosine kinase non receptor 2 Tyrosine kinase non-receptor protein 2 non-receptor protein tyrosine kinase ACK ACK TNK 2 Tnk2 ACK1 FLJ44758 FLJ45547 ACK 1 ACK-1 ACK1_HUMAN Activated p21cdc42Hs kinase FLJ44758 FLJ45547 Tyrosine kinase non receptor protein 2 p21cdc42Hs. |
| Product description | Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. |
| Immunogen | KLH conjugated synthetic peptide derived from human Ack1 301-420/1055 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCow |
| Concentration | 1mg/ml |
| Alternative Names | Acetate kinase 1 Acetokinase 1 Activated CDC42 kinase 1 Activated Cdc42 associated kinase 1 Activated p21cdc42Hs kinase Tyrosine kinase non receptor 2 Tyrosine kinase non-receptor protein 2 non-receptor protein tyrosine kinase ACK ACK TNK 2 Tnk2 ACK1 FLJ44758 FLJ45547 ACK 1 ACK-1 ACK1_HUMAN Activated p21cdc42Hs kinase FLJ44758 FLJ45547 Tyrosine kinase non receptor protein 2 p21cdc42Hs. |
|---|---|
| Molecular Weight(MW) | 119 KDa |
| Cellular Localization | Cell membrane. Nucleus. Endosome. Cell junction, adherens junction. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle. Membrane, clathrin-coated pit. Note=The Tyr-284 phosphorylated form is found both in the membrane and nucleus. Co-localizes with EGFR on endosomes. Nuclear translocation is CDC42-dependent. |
| SwissProt ID | Q07912 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ack1) Polyclonal Antibody, Unconjugated (ER1903-20) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Spinal cord(Mouse)l Lysate at 40 ug Primary: Anti-Ack1 (ER1903-20) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 119 kD Observed band size:119 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ack1) Polyclonal Antibody, Unconjugated (ER1903-20) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | The Tyr-284 phosphorylated form shows asignificant increase in expression in breast cancers during theprogressive stages i.e. normal to hyperplasia (ADH), ductalcarcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymphnode metastatic (LNMM) stages. It also shows a significant increasein expression in prostate cancers during the progressive stages. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
