Application
Fig1: Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-ADAM12 antibody (ER1903-25) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-ADAM12 (ER1903-25) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77/100 kD Observed band size: 115 kD| Product Name | ADAM12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A disintegrin and metalloproteinase domain 12 A disintegrin and metalloproteinase domain 12 ADA12_HUMAN ADAM 12 ADAM metallopeptidase domain 12 Disintegrin and metalloproteinase domain-containing protein 12 MCMP MCMPMltna MCMPMltna Meltrin alpha Meltrin-alpha MLTN MLTNA OTTHUMP00000046766. |
| Product description | Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human ADAM12 201-300/909 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | A disintegrin and metalloproteinase domain 12 A disintegrin and metalloproteinase domain 12 ADA12_HUMAN ADAM 12 ADAM metallopeptidase domain 12 Disintegrin and metalloproteinase domain-containing protein 12 MCMP MCMPMltna MCMPMltna Meltrin alpha Meltrin-alpha MLTN MLTNA OTTHUMP00000046766. |
|---|---|
| Molecular Weight(MW) | 77/100 KDa |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein.;Isoform 2: Secreted.;Isoform 3: Secreted (Potential).;Isoform 4: Secreted (Potential). |
| SwissProt ID | O43184 |
|---|
Application
Fig1: Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-ADAM12 antibody (ER1903-25) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-ADAM12 (ER1903-25) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77/100 kD Observed band size: 115 kD| Positive Control | Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
