Application
Fig1: Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-ADAM23 antibody (ER1903-30) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Raji Cell (Human) Lysate at 40 ug Primary: Anti-ADAM23 (ER1903-30) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 75-95kD Observed band size: 75/35 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADAM23) Polyclonal Antibody, Unconjugated (ER1903-30) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | ADAM23 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A disintegrin and metalloproteinase domain 23 MDC 3 Metalloproteinase like disintegrin like and cysteine rich protein 3. |
| Immunogen | KLH conjugated synthetic peptide derived from human ADAM23 551-650/832 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:0.2ug/Test: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | A disintegrin and metalloproteinase domain 23 MDC 3 Metalloproteinase like disintegrin like and cysteine rich protein 3. |
|---|---|
| Molecular Weight(MW) | 75-95 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Isoform Gamma: Secreted. |
| SwissProt ID | O75077 |
|---|
Application
Fig1: Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-ADAM23 antibody (ER1903-30) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Raji Cell (Human) Lysate at 40 ug Primary: Anti-ADAM23 (ER1903-30) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 75-95kD Observed band size: 75/35 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADAM23) Polyclonal Antibody, Unconjugated (ER1903-30) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Highly expressed in the brain and weakly expressed in the heart. In the brain, expressed prominently in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:0.2ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
