Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated (ER1903-32) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample:Liver (Mouse) Lysate at 40 ug Primary: Anti-ADAMTS5 (ER1903-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 102 kD| Product Name | ADAMTS5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADAMTS5 A disintegrin and metalloproteinase with thrombospondin motifs 5 A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 ADAM metallopeptidase with thrombospondin type 1 motif 5 ADAM TS 11 ADAM TS 5 ADAM TS5 ADAMTS 11 ADAMTS 5 ADAMTS11 ADMP 2 ADMP2 Aggrecanase 2 aggrecanase-2 FLJ36738 Implantin ThromboSpondin motif-5. |
| Product description | Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan (PubMed:16133547, PubMed:18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its versican remodeling properties. Participates in development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection. |
| Immunogen | KLH conjugated synthetic peptide derived from human Aggrecan 2 511-610/930 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | ADAMTS5 A disintegrin and metalloproteinase with thrombospondin motifs 5 A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 ADAM metallopeptidase with thrombospondin type 1 motif 5 ADAM TS 11 ADAM TS 5 ADAM TS5 ADAMTS 11 ADAMTS 5 ADAMTS11 ADMP 2 ADMP2 Aggrecanase 2 aggrecanase-2 FLJ36738 Implantin ThromboSpondin motif-5. |
|---|---|
| Molecular Weight(MW) | 102 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | Q9UNA0 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated (ER1903-32) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample:Liver (Mouse) Lysate at 40 ug Primary: Anti-ADAMTS5 (ER1903-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 102 kD| Positive Control | Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
