Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated(ER1903-33) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADAMTS7(NT)) Polyclonal Antibody, Unconjugated (ER1903-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- ADAMTS7(NT) (ER1903-33) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 158 kD Observed band size: 158 kD| Product Name | ADAMTS7(NT) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A disintegrin and metalloproteinase with thrombospondin motifs 7 ADAM metallopeptidase with thrombospondin type 1 motif 7 A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein A disintegrin and metalloproteinase with thrombospondin motifs 7 A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 A disintegrin like and metalloprotease with thrombospondin type 1 motif 7 ADAM metallopeptidase with thrombospondin type 1 motif 7 ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein ADAM TS 7 ADAM TS7 ADAMTS 7 ADAMTS7 COMPase DKFZp434H204 ATS7_MOUSE. |
| Product description | Metalloprotease that may play a role in the degradation of COMP. |
| Immunogen | KLH conjugated synthetic peptide derived from rat ADAMTS7 241-340/1595 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | A disintegrin and metalloproteinase with thrombospondin motifs 7 ADAM metallopeptidase with thrombospondin type 1 motif 7 A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein A disintegrin and metalloproteinase with thrombospondin motifs 7 A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 A disintegrin like and metalloprotease with thrombospondin type 1 motif 7 ADAM metallopeptidase with thrombospondin type 1 motif 7 ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein ADAM TS 7 ADAM TS7 ADAMTS 7 ADAMTS7 COMPase DKFZp434H204 ATS7_MOUSE. |
|---|---|
| Molecular Weight(MW) | 158 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. Note=Also found associated with the external cell surface. |
| SwissProt ID | Q68SA9 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated(ER1903-33) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADAMTS7(NT)) Polyclonal Antibody, Unconjugated (ER1903-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- ADAMTS7(NT) (ER1903-33) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 158 kD Observed band size: 158 kD| Positive Control | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
