Application
Fig1: Western blot analysis of Nicastrin on THP-1 cell lysate using anti-Nicastrin antibody at 1/1,000 dilution.| Product Name | Nicastrin [JG68-33] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Anterior pharynx defective 2 antibody APH 2 antibody APH2 antibody ATAG1874 antibody KIAA0253 antibody Ncstn antibody NCT antibody NICA_HUMAN antibody Nicastrin antibody RP11 517F10.1 antibody RP11517F101 antibody |
| Product description | The Presenilin 1 (PS1) and Presenilin 2 (PS2) transmembrane proteins are components of high molecular weight complexes. These complexes mediate proteolytic cleavage within the transmembrane domain of several proteins, including the β-Amyloid precursor protein (βAPP) and Notch. Missense mutations in the genes encoding the Presenilin proteins increase the proteolysis of βAPP and results in the overproduction of the neurotoxic β-Amyloid peptide, which results in a condition associated with Familial Alzheimer's disease (FAD). A novel component of the presenilin complex, nicastrin, is a type I transmembrane glycoprotein that is involved in mediating Notch/GLP-1 signaling. In addition, nicastrin contributes to the processing of βAPP, which makes nicastrin an attractive potential target for modulating the production of β-Amyloid in patients with Alzheimer's disease. Originally purified from immunoprecipitated PS1 complexes from HEK293 cells, nicastrin contains hydrophilic amino and carboxy-terminal domains, a short, hydrophobic transmembrane domain and potential N-myristoylation and phosphorylation sites. |
| Immunogen | Synthetic peptide within Human Nicastrin aa 650-750 (C terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:1,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Anterior pharynx defective 2 antibody APH 2 antibody APH2 antibody ATAG1874 antibody KIAA0253 antibody Ncstn antibody NCT antibody NICA_HUMAN antibody Nicastrin antibody RP11 517F10.1 antibody RP11517F101 antibody |
|---|---|
| Molecular Weight(MW) | 78 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q92542 |
|---|
Application
Fig1: Western blot analysis of Nicastrin on THP-1 cell lysate using anti-Nicastrin antibody at 1/1,000 dilution.| Positive Control | THP-1. |
|---|---|
| Application Notes | WB:1:500-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
