WB
Western blot analysis of SOCS2 on different lysates with Rabbit anti-SOCS2 antibody at 1/1,000 dilution. Lane 1: SH-SY-5Y cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 2: PC-12 cell lysate, Lane 3: Mouse thymus tissue lysate, Lane 4: Rat thymus tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibod at 1:50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-SOCS2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining SOCS2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining SOCS2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | SOCS2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
| Immunogen | Recombinant protein within Human SOCS2 aa 1-198 / 198. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Purification | Protein A |
| Alternative Names | CIS 2 antibody CIS-2 antibody CIS2 antibody Cish 2 antibody Cish2 antibody Cytokine inducible SH2 protein 2 antibody Cytokine-inducible SH2 protein 2 antibody SOCS 2 antibody SOCS-2 antibody Socs2 antibody SOCS2_HUMAN antibody SSI 2 antibody SSI-2 antibody SSI2 antibody STAT induced STAT inhibitor 2 antibody STAT-induced STAT inhibitor 2 antibody STATI 2 antibody STATI2 antibody Suppressor of cytokine signaling 2 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytosol. |
| SwissProt ID | O14508 |
|---|
WB
Western blot analysis of SOCS2 on different lysates with Rabbit anti-SOCS2 antibody at 1/1,000 dilution. Lane 1: SH-SY-5Y cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 2: PC-12 cell lysate, Lane 3: Mouse thymus tissue lysate, Lane 4: Rat thymus tissue lysate, Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibod at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-SOCS2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining SOCS2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining SOCS2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Application Notes | WB:1:500-1:2000 IHC:1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
