Application
Fig1: Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃). Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody(ER1903-40); Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.Application
Fig2: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated(ER1903-40) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: Colon carcinoma lysates (Human) Lysate at 40 ug Gastric carcinoma lysates (Human) Lysate at 40 ug Primary: Anti- ADORA3 (ER1903-40) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 35 kD Observed band size: 35 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (ER1903-40) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | Adenosine A3 Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | adenosine A3 receptor A3AR AD 026 AD026 ADORA 3 ADORA3 bA552M11.5 RP11 552M11.7 TGPCR1 adenosine receptor A3 isoform 1 adenosine receptor A3 isoform 2 AA3R_HUMAN. |
| Product description | Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction. |
| Immunogen | KLH conjugated synthetic peptide derived from human ADORA3 85-180/347 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | adenosine A3 receptor A3AR AD 026 AD026 ADORA 3 ADORA3 bA552M11.5 RP11 552M11.7 TGPCR1 adenosine receptor A3 isoform 1 adenosine receptor A3 isoform 2 AA3R_HUMAN. |
|---|---|
| Molecular Weight(MW) | 35 KDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P33765 |
|---|
Application
Fig1: Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃). Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody(ER1903-40); Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.
Application
Fig2: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated(ER1903-40) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: Colon carcinoma lysates (Human) Lysate at 40 ug Gastric carcinoma lysates (Human) Lysate at 40 ug Primary: Anti- ADORA3 (ER1903-40) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 35 kD Observed band size: 35 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (ER1903-40) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
