Application
Fig1: Western blot analysis of WNK1 on A549 cell lysates using anti-WNK1 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-WNK1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-WNK1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-WNK1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-WNK1 antibody. Counter stained with hematoxylin.| Product Name | WNK1 [JG36-91] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Erythrocyte 65 kDa protein antibody HSAN2 antibody HSN2 antibody hWNK1 antibody KDP antibody KIAA0344 antibody Kinase deficient protein antibody MGC163339 antibody MGC163341 antibody p65 antibody PPP1R167 antibody PRKWNK1 antibody Prostate derived sterile 20 like kinase antibody Protein kinase lysine deficient 1 antibody Protein kinase lysine-deficient 1 antibody Protein kinase with no lysine 1 antibody Protein phosphatase 1, regulatory subunit 167 antibody PSK antibody Serine/threonine protein kinase WNK1 1 antibody Serine/threonine protein kinase WNK1 2 antibody Serine/threonine protein kinase WNK1 antibody Serine/threonine-protein kinase WNK1 antibody With no K antibody WNK lysine deficient protein kinase 1 antibody WNK lysine deficient protein kinase 1 isoform antibody WNK1 antibody WNK1_HUMAN antibody |
| Product description | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition. |
| Immunogen | Synthetic peptide within Human WNK1 aa 100-200 (Cysteine residue). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Erythrocyte 65 kDa protein antibody
HSAN2 antibody
HSN2 antibody
hWNK1 antibody
KDP antibody
KIAA0344 antibody
Kinase deficient protein antibody
MGC163339 antibody
MGC163341 antibody
p65 antibody
PPP1R167 antibody
PRKWNK1 antibody
Prostate derived sterile 20 like kinase antibody
Protein kinase lysine deficient 1 antibody
Protein kinase lysine-deficient 1 antibody
Protein kinase with no lysine 1 antibody
Protein phosphatase 1 regulatory subunit 167 antibody PSK antibody Serine/threonine protein kinase WNK1 1 antibody Serine/threonine protein kinase WNK1 2 antibody Serine/threonine protein kinase WNK1 antibody Serine/threonine-protein kinase WNK1 antibody With no K antibody WNK lysine deficient protein kinase 1 antibody WNK lysine deficient protein kinase 1 isoform antibody WNK1 antibody WNK1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 250/300 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of WNK1 on A549 cell lysates using anti-WNK1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-WNK1 antibody. Counter stained with hematoxylin.| Positive Control | A549, Rat kidney tissue, human skin tissue, human breast cancer tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
