Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (ER1903-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-ADORA2B (ER1903-44) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 36 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (ER1903-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig4: Blank control: A549. Primary Antibody (green line): Rabbit Anti-ADORA2B antibody (ER1903-44) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | ADORA2B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenosine A2b Receptor adora 2 adora 2b ADORA2 ADORA2B HGNC:264 Netrin 1 receptor AA2BR_HUMAN. |
| Product description | Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. |
| Immunogen | KLH conjugated synthetic peptide derived from human ADORA2B 101-200/332 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IFFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/test: IF:1:100-500: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Adenosine A2b Receptor adora 2 adora 2b ADORA2 ADORA2B HGNC:264 Netrin 1 receptor AA2BR_HUMAN. |
|---|---|
| Molecular Weight(MW) | 37 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P29275 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (ER1903-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-ADORA2B (ER1903-44) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 36 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (ER1903-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig4: Blank control: A549. Primary Antibody (green line): Rabbit Anti-ADORA2B antibody (ER1903-44) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/test: IF:1:100-500: ICC:1:100-500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
