Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-ADRA1A (ER1903-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 62 kDApplication
Fig2: Blank control: U-87MG(blue). Primary Antibody:Rabbit Anti-ADRA1A antibody(ER1903-45), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1903-45,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig3: Sample: U937 Cell (Human) Lysate at 30 ug Raji Cell (Human) Lysate at 30 ug Primary: Anti-ADRA1A (ER1903-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 55 kDApplication
Fig4: Sample: kidney (Mouse) Lysate at 40 ug Primary: Anti-ADRA1A (ER1903-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 62 kD| Product Name | ADRA1A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADA1A_HUMAN Adrenergic alpha 1A receptor Adrenergic alpha 1C receptor Adrenergic alpha 1D receptor alpha 1 Adrenergic Receptor Alpha 1A adrenergic receptor Alpha-1A adrenergic receptor Alpha-1A adrenoreceptor Alpha-1C adrenergic receptor Alpha-adrenergic receptor 1c ADRA1A ADRA1C Alpha 1A adrenoceptor alpha-1A adrenergic receptor isoform 1 adrenergic, alpha-1A-, receptor variant 1 adrenergic, alpha-1A-, receptor variant 3 adrenergic, alpha-1A-, receptor variant 5 adrenergic, alpha-1A-, receptor variant 8 G protein coupled receptor alpha-1A adrenoceptor ADRA1L1 ALPHA1AAR. |
| Product description | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. |
| Immunogen | KLH conjugated synthetic peptide derived from human Alpha-1A adrenergic receptor 201-300/466 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | ADA1A_HUMAN
Adrenergic alpha 1A receptor
Adrenergic alpha 1C receptor
Adrenergic alpha 1D receptor
alpha 1 Adrenergic Receptor
Alpha 1A adrenergic receptor
Alpha-1A adrenergic receptor
Alpha-1A adrenoreceptor
Alpha-1C adrenergic receptor
Alpha-adrenergic receptor 1c
ADRA1A
ADRA1C
Alpha 1A adrenoceptor
alpha-1A adrenergic receptor isoform 1
adrenergic alpha-1A- receptor variant 1 adrenergic alpha-1A- receptor variant 3 adrenergic alpha-1A- receptor variant 5 adrenergic alpha-1A- receptor variant 8 G protein coupled receptor alpha-1A adrenoceptor ADRA1L1 ALPHA1AAR. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. |
| SwissProt ID | P35348 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-ADRA1A (ER1903-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 62 kD
Application
Fig2: Blank control: U-87MG(blue). Primary Antibody:Rabbit Anti-ADRA1A antibody(ER1903-45), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1903-45,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: U937 Cell (Human) Lysate at 30 ug Raji Cell (Human) Lysate at 30 ug Primary: Anti-ADRA1A (ER1903-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 55 kD
Application
Fig4: Sample: kidney (Mouse) Lysate at 40 ug Primary: Anti-ADRA1A (ER1903-45) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 62 kD| Positive Control | Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
