Application
Fig1: Blank control: U-2OS(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).Application
Fig2: Sample: Testis (Rat) Lysate at 40 ug Primary: Anti-AF10 (ER1903-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 109 kD Observed band size: 109 kD| Product Name | AF10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AF 10 AF10 AF10_HUMAN ALL 1 fused gene from chromosome 10 ALL1 fused gene from chromosome 10 ALL1-fused gene from chromosome 10 protein MLLT 10 MLLT10 Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10 Myeloid/lymphoid or mixed lineage leukemia translocated to 10 Protein AF 10 Protein AF-10 Protein AF10 Type I AF10 protein. |
| Product description | Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. |
| Immunogen | KLH conjugated synthetic peptide derived from human AF10/MLLT10 151-250/1027 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | AF 10 AF10 AF10_HUMAN ALL 1 fused gene from chromosome 10 ALL1 fused gene from chromosome 10 ALL1-fused gene from chromosome 10 protein MLLT 10 MLLT10 Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10 Myeloid/lymphoid or mixed lineage leukemia translocated to 10 Protein AF 10 Protein AF-10 Protein AF10 Type I AF10 protein. |
|---|---|
| Molecular Weight(MW) | 109 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P55197 |
|---|
Application
Fig1: Blank control: U-2OS(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Application
Fig2: Sample: Testis (Rat) Lysate at 40 ug Primary: Anti-AF10 (ER1903-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 109 kD Observed band size: 109 kD| Positive Control | Expressed abundantly in testis. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
