Application
Fig1: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-Afadin (ER1903-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kD Observed band size: 135/138 kDApplication
Fig2: Sample: Skin (Rat) Lysate at 40 ug Primary: Anti-Afadin (ER1903-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kD Observed band size: 135/138 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Afadin) Polyclonal Antibody, Unconjugated (ER1903-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Afadin/I Afadin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AF 6 AF6 AFAD_HUMAN Afadin ALL1-fused gene from chromosome 6 protein FLJ34371 MLLT 4 MLLT4 Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 4 Myeloid/lymphoid or mixed lineage leukemia translocated to 4 Protein AF 6 Protein Af-6 Protein AF6 RP3 431P23.3 5033403D15Rik Gm314 I-afadin Mllt4 S-afadin. |
| Product description | Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. |
| Immunogen | KLH conjugated synthetic peptide derived from human AF6 341-440/1816 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | AF 6 AF6 AFAD_HUMAN Afadin ALL1-fused gene from chromosome 6 protein FLJ34371 MLLT 4 MLLT4 Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 4 Myeloid/lymphoid or mixed lineage leukemia translocated to 4 Protein AF 6 Protein Af-6 Protein AF6 RP3 431P23.3 5033403D15Rik Gm314 I-afadin Mllt4 S-afadin. |
|---|---|
| Molecular Weight(MW) | 136 KDa |
| Cellular Localization | Cell junction, adherens junction. Note=Not found at cell-matrix AJs. |
| SwissProt ID | P55196 |
|---|
Application
Fig1: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-Afadin (ER1903-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kD Observed band size: 135/138 kD
Application
Fig2: Sample: Skin (Rat) Lysate at 40 ug Primary: Anti-Afadin (ER1903-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kD Observed band size: 135/138 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Afadin) Polyclonal Antibody, Unconjugated (ER1903-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
