Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (AGPAT1) Polyclonal Antibody, Unconjugated (ER1903-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Sample: Siver (Mouse) Lysate at 40 ug Primary: Anti-AGPAT1 (ER1903-53) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD| Product Name | AGPAT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1 AGPAT1 LPAAT alpha LPAATA MGC5423 1 acyl sn glycerol 3 phosphate acyltransferase alpha 1 acylglycerol 3 phosphate O acyltransferase 1 (acetoacetly Coenzyme A thiolase) 1 acylglycerol 3 phosphate O acyltransferase 1 (lysophosphatidic acid acyltransferase alpha) 1 acylglycerol 3 phosphate O acyltransferase 1 1 AGP acyltransferase 1 AGPAT 1 G15 antibody Lysophosphatidic acid acyltransferase alpha Lysophospholipid acyltransferase MGC4007 OTTHUMP00000062693 Protein G15 Protein-G15. PLCA_HUMAN |
| Product description | Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. |
| Immunogen | KLH conjugated synthetic peptide derived from human AGPAT1 101-200/283 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | 1 AGPAT1 LPAAT alpha LPAATA MGC5423 1 acyl sn glycerol 3 phosphate acyltransferase alpha 1 acylglycerol 3 phosphate O acyltransferase 1 (acetoacetly Coenzyme A thiolase) 1 acylglycerol 3 phosphate O acyltransferase 1 (lysophosphatidic acid acyltransferase alpha) 1 acylglycerol 3 phosphate O acyltransferase 1 1 AGP acyltransferase 1 AGPAT 1 G15 antibody Lysophosphatidic acid acyltransferase alpha Lysophospholipid acyltransferase MGC4007 OTTHUMP00000062693 Protein G15 Protein-G15. PLCA_HUMAN |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein |
| SwissProt ID | Q99943 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (AGPAT1) Polyclonal Antibody, Unconjugated (ER1903-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Sample: Siver (Mouse) Lysate at 40 ug Primary: Anti-AGPAT1 (ER1903-53) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
