Application
Fig1: Sample: Raw264.7 Cell Lysate at 40 ug A431 Cell Lysate at 40 ug Primary: Anti-ALOX12 (ER1903-58) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kD Observed band size: 73 kD| Product Name | ALOX12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 12 Lox 12(S) lipoxygenase 12S LOX Alox 12 Alox12L Alox15 Arachidonate 12 lipoxygenase 12S type Arachidonate 12 lipoxygenase Arachidonate 12 lipoxygenase leukocyte type Arachidonate 12 lipoxygenase, 12S type Arachidonate 12 lipoxygenase, leukocyte type EC1.13.11.31 LOG 12 LOG12 Platelet type 12 lipoxygenase Platelet type lipoxygenase 12 P-12-LOX LOX12_HUMAN 12 Lipoxygenase. |
| Product description | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO:0000269|PubMed:16638750, |
| Immunogen | KLH conjugated synthetic peptide derived from human 12 Lipoxygenase 101-200/663 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCow |
| Concentration | 1mg/ml |
| Alternative Names | 12 Lox
12(S) lipoxygenase
12S LOX
Alox 12
Alox12L
Alox15
Arachidonate 12 lipoxygenase 12S type
Arachidonate 12 lipoxygenase
Arachidonate 12 lipoxygenase leukocyte type
Arachidonate 12 lipoxygenase 12S type Arachidonate 12 lipoxygenase leukocyte type EC1.13.11.31 LOG 12 LOG12 Platelet type 12 lipoxygenase Platelet type lipoxygenase 12 P-12-LOX LOX12_HUMAN 12 Lipoxygenase. |
|---|---|
| Molecular Weight(MW) | 76 KDa |
| Cellular Localization | Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF. |
| SwissProt ID | P18054 |
|---|
Application
Fig1: Sample: Raw264.7 Cell Lysate at 40 ug A431 Cell Lysate at 40 ug Primary: Anti-ALOX12 (ER1903-58) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kD Observed band size: 73 kD| Positive Control | Expressed in vascular smooth muscle cells. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
