Application
Fig1: Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution.Application
Fig2: ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HIF1AN in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-HIF1AN antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | HIF1AN [JG37-66] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp762F1811 antibody Factor inhibiting HIF-1 antibody Factor inhibiting HIF1 antibody FIH 1 antibody FIH-1 antibody FIH1 antibody FLJ20615 antibody FLJ22027 antibody HIF1AN antibody HIF1N_HUMAN antibody Hypoxia inducible factor 1 alpha inhibitor antibody Hypoxia inducible factor 1 alpha subunit inhibitor antibody Hypoxia inducible factor asparagine hydroxylase antibody Hypoxia-inducible factor 1-alpha inhibitor antibody Hypoxia-inducible factor asparagine hydroxylase antibody Peptide aspartate beta dioxygenase antibody |
| Product description | Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1α. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the β-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1α. This hydroxylation event blocks the association of HIF-1α with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1α. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like β-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24. |
| Immunogen | Recombinant protein within human HIF1AN aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp762F1811 antibody Factor inhibiting HIF-1 antibody Factor inhibiting HIF1 antibody FIH 1 antibody FIH-1 antibody FIH1 antibody FLJ20615 antibody FLJ22027 antibody HIF1AN antibody HIF1N_HUMAN antibody Hypoxia inducible factor 1 alpha inhibitor antibody Hypoxia inducible factor 1 alpha subunit inhibitor antibody Hypoxia inducible factor asparagine hydroxylase antibody Hypoxia-inducible factor 1-alpha inhibitor antibody Hypoxia-inducible factor asparagine hydroxylase antibody Peptide aspartate beta dioxygenase antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
| SwissProt ID | Q9NWT6 |
|---|
Application
Fig1: Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution.
Application
Fig2: ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HIF1AN in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-HIF1AN antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, LOVO, SiHa, human prostate cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
