Application
Fig1: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-alpha Adaptin (ER1903-59) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103 kD Observed band size: 103 kDApplication
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-alpha Adaptin (ER1903-59) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103 kD Observed band size: 103 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (alpha Adaptin) Polyclonal Antibody, Unconjugated (ER1903-59) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | alpha Adaptin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 100 kDa coated vesicle protein A 100 kDa coated vesicle protein C adaptin alpha A Adaptin alpha B Adaptor protein complex AP 2 alpha 1 subunit Adaptor protein complex AP 2 alpha 2 subunit Adaptor related protein complex 2 alpha 1 subunit Adaptor related protein complex 2 alpha 2 subunit ADTAA ADTAB Alpha adaptin A Alpha adaptin C AP 2 complex subunit alpha 1 AP 2 complex subunit alpha 2 AP2A2 CLAPA1 CLAPA2 Clathrin assembly protein complex 2 alpha A large chain Clathrin assembly protein complex 2 alpha C large chain Clathrin associated/assembly/adaptor protein large alpha 1 Clathrin associated/assembly/adaptor protein large alpha 2 HIP9 Huntingtin interacting protein HYPJ Huntingtin interacting protein J HYPJ KIAA0899 Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit AP2A2_HUMAN AP-2 complex subunit alpha-2 Adapter-related protein complex 2 alpha-2 subunit Adaptor protein complex AP-2 subunit alpha-2 Alpha-adaptin C Alpha2-adaptin Clathrin assembly protein complex 2 alpha-C large chain Huntingtin yeast partner J Huntingtin-interacting protein 9 HIP-9 Huntingtin-interacting protein J Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit. |
| Product description | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. |
| Immunogen | KLH conjugated synthetic peptide derived from human alpha Adaptin 21-150/939 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 100 kDa coated vesicle protein A 100 kDa coated vesicle protein C adaptin alpha A Adaptin alpha B Adaptor protein complex AP 2 alpha 1 subunit Adaptor protein complex AP 2 alpha 2 subunit Adaptor related protein complex 2 alpha 1 subunit Adaptor related protein complex 2 alpha 2 subunit ADTAA ADTAB Alpha adaptin A Alpha adaptin C AP 2 complex subunit alpha 1 AP 2 complex subunit alpha 2 AP2A2 CLAPA1 CLAPA2 Clathrin assembly protein complex 2 alpha A large chain Clathrin assembly protein complex 2 alpha C large chain Clathrin associated/assembly/adaptor protein large alpha 1 Clathrin associated/assembly/adaptor protein large alpha 2 HIP9 Huntingtin interacting protein HYPJ Huntingtin interacting protein J HYPJ KIAA0899 Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit AP2A2_HUMAN AP-2 complex subunit alpha-2 Adapter-related protein complex 2 alpha-2 subunit Adaptor protein complex AP-2 subunit alpha-2 Alpha-adaptin C Alpha2-adaptin Clathrin assembly protein complex 2 alpha-C large chain Huntingtin yeast partner J Huntingtin-interacting protein 9 HIP-9 Huntingtin-interacting protein J Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit. |
|---|---|
| Molecular Weight(MW) | 103 KDa |
| Cellular Localization | Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. |
| SwissProt ID | O94973 |
|---|
Application
Fig1: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-alpha Adaptin (ER1903-59) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103 kD Observed band size: 103 kD
Application
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-alpha Adaptin (ER1903-59) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103 kD Observed band size: 103 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (alpha Adaptin) Polyclonal Antibody, Unconjugated (ER1903-59) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
