Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Alpha-ENaC (ER1903-62) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 77 kDApplication
Fig2: Sample: Kidney (Rat) Lysate at 40 ug Primary: Anti-Alpha-ENaC (ER1903-62) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 77 kDApplication
Fig3: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated(ER1903-62) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and . Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μg in 100 μL1X PBS containing 0.5% BSA(green).| Product Name | Alpha-ENaC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha ENaC 2 Alpha ENaC Alpha NaCH Alpha-ENaC Alpha-NaCH Amiloride sensitive epithelial sodium channel alpha subunit Amiloride sensitive sodium channel subunit alpha Amiloride-sensitive sodium channel subunit alpha ENaCa ENaCalpha Epithelial Na(+) channel subunit alpha Epithelial Na+ channel subunit alpha FLJ21883 Nonvoltage gated sodium channel 1 subunit alpha Nonvoltage-gated sodium channel 1 subunit alpha SCNEA SCNN 1 SCNN1 SCNN1A SCNNA_HUMAN Sodium channel nonvoltage gated 1 alpha. |
| Product description | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. |
| Immunogen | KLH conjugated synthetic peptide derived from human Alpha-ENaC 201-300/669 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:5ug/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Alpha ENaC 2 Alpha ENaC Alpha NaCH Alpha-ENaC Alpha-NaCH Amiloride sensitive epithelial sodium channel alpha subunit Amiloride sensitive sodium channel subunit alpha Amiloride-sensitive sodium channel subunit alpha ENaCa ENaCalpha Epithelial Na(+) channel subunit alpha Epithelial Na+ channel subunit alpha FLJ21883 Nonvoltage gated sodium channel 1 subunit alpha Nonvoltage-gated sodium channel 1 subunit alpha SCNEA SCNN 1 SCNN1 SCNN1A SCNNA_HUMAN Sodium channel nonvoltage gated 1 alpha. |
|---|---|
| Molecular Weight(MW) | 76 KDa |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P37088 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Alpha-ENaC (ER1903-62) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 77 kD
Application
Fig2: Sample: Kidney (Rat) Lysate at 40 ug Primary: Anti-Alpha-ENaC (ER1903-62) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 77 kD
Application
Fig3: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated(ER1903-62) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and . Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μg in 100 μL1X PBS containing 0.5% BSA(green).| Positive Control | Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:5ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
