Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-AMHR2 (ER1903-64) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 63 kDApplication
Fig2: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- AMHR2 (ER1903-64)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61 kDApplication
Fig3: Blank control(blue): Jurkat cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-AMHR2 antibody antibody(ER1903-64), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | AMHR2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMH type II receptor AMHR2 AMHR2_HUMAN Anti-Muellerian hormone type II receptor Anti-Muellerian hormone type-2 receptor MGC141312 MIS type II receptor MISRII MRII Muellerian hormone type 2 receptor Muellerian hormone type II receptor Mullerian hormone receptor type II. |
| Product description | On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone. |
| Immunogen | KLH conjugated synthetic peptide derived from human MISRII/AMHR2 21-120/573 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigHorse |
| Concentration | 1mg/ml |
| Alternative Names | AMH type II receptor AMHR2 AMHR2_HUMAN Anti-Muellerian hormone type II receptor Anti-Muellerian hormone type-2 receptor MGC141312 MIS type II receptor MISRII MRII Muellerian hormone type 2 receptor Muellerian hormone type II receptor Mullerian hormone receptor type II. |
|---|---|
| Molecular Weight(MW) | 61 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q16671 |
|---|
Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-AMHR2 (ER1903-64) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 63 kD
Application
Fig2: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- AMHR2 (ER1903-64)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61 kD
Application
Fig3: Blank control(blue): Jurkat cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-AMHR2 antibody antibody(ER1903-64), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
