Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (ER1903-66) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AMIGO2 Polyclonal Antibody, Unconjugated(ER1903-66) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- AMIGO2 (ER1903-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Product Name | AMIGO2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adhesion molecule with Ig like domain 2 ALI 1 ALI1 Alivin 1 Alivin-1 Alivin1 AMIGO 2 Amphoterin induced gene 2 Amphoterin induced protein 2 DEGA AMGO2_HUMAN AMIGO-2 Amigo2 Amphoterin induced protein 2 Amphoterin-induced protein 2 Differentially expressed in gastric adenocarcinoma Differentially expressed in gastric adenocarcinomas Transmembrane protein AMIGO 2 Transmembrane protein AMIGO2. |
| Product description | A novel primary response gene, AMIGO2, is an activity-dependent gene and promotes survival of neurons. Sequence analyses reveal that rat, mouse, and human AMIGO2 proteins contain seven leucine-rich repeats, one IgC2-like loop and a transmembrane domain, and display homology to Kek and Trk families. Results suggest that expression of ali1 promotes depolarization-dependent survival of the granule neuron. Mouse AMIGO2 was mapped to a locus approximately 55.3 cm from the centromere on chromosome 15 that is syntenic to positional candidate loci for familial Alzheimer's disease type 5 and Parkinson's disease 8 on human chromosome 12. |
| Immunogen | KLH conjugated synthetic peptide derived from human AMIGO2 21-120/522 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Adhesion molecule with Ig like domain 2 ALI 1 ALI1 Alivin 1 Alivin-1 Alivin1 AMIGO 2 Amphoterin induced gene 2 Amphoterin induced protein 2 DEGA AMGO2_HUMAN AMIGO-2 Amigo2 Amphoterin induced protein 2 Amphoterin-induced protein 2 Differentially expressed in gastric adenocarcinoma Differentially expressed in gastric adenocarcinomas Transmembrane protein AMIGO 2 Transmembrane protein AMIGO2. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Nucleus. Note: Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons By |
| SwissProt ID | Q86SJ2 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (ER1903-66) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AMIGO2 Polyclonal Antibody, Unconjugated(ER1903-66) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- AMIGO2 (ER1903-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Positive Control | Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
