Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Amphiregulin (ER1903-67) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 41 kDApplication
Fig2: Blank control:A549. Primary Antibody (green line): Rabbit Anti-Amphiregulin antibody (ER1903-67) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Amphiregulin (ER1903-67) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 41 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (ER1903-67) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | Amphiregulin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AR Colorectum cell derived growth factor CRDGF A REG AR antibody AREG Colorectum cell derived growth factor CRDGF MGC13647 Schwannoma derived growth factor SDGF AREG_HUMAN. |
| Product description | Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. |
| Immunogen | KLH conjugated synthetic peptide derived from human Amphiregulin 185-252/252 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1:ug/test: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | AR Colorectum cell derived growth factor CRDGF A REG AR antibody AREG Colorectum cell derived growth factor CRDGF MGC13647 Schwannoma derived growth factor SDGF AREG_HUMAN. |
|---|---|
| Molecular Weight(MW) | 16 KDa |
| Cellular Localization | Membrane; Single-pass membrane protein. |
| SwissProt ID | P15514 |
|---|
Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Amphiregulin (ER1903-67) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 41 kD
Application
Fig2: Blank control:A549. Primary Antibody (green line): Rabbit Anti-Amphiregulin antibody (ER1903-67) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Amphiregulin (ER1903-67) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 41 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (ER1903-67) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1:ug/test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
