Application
Fig1: Positive control(blue): mouse Splenocytes
Isotype Antibody (orenge): Rabbit IgG, Dilution: 1ug in 100 u1 X PBS containing 0.5% BSA
Secondary Antibody(light blue): Goat anti-rabbit IgG-FITC,Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody catalog number (green): ER1903-75, Dilution: 1ug in 100ul 1X PBS containing 0.5% BSA
Application
Fig2: Sample:
Cerebrum(mouse) Lysate at 40 ug
heart(mouse) Lysate at 40 ug
Primary: Anti-Apelin receptor (ER1903-75) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 52 kD