Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-APEX2 Polyclonal Antibody, Unconjugated(ER1903-76) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Lymph node(Mouse) Lysate at 40 ug Primary: Anti- APEX2 (ER1903-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 57kDApplication
Fig3: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- APEX2 (ER1903-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 57kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (APEX2) Polyclonal Antibody, Unconjugated (ER1903-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (APEX2) Polyclonal Antibody, Unconjugated (ER1903-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | APEX2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP endonuclease 2 AP endonuclease XTH2 APE 2 APE2 APEX 2 APEX L2 APEX nuclease (apurinic/apyrimidinic endonuclease) 2 APEX Nuclease 2 APEX nuclease like 2 APEXL 2 APEXL2 Apurinic apyrimidinic endonuclease 2 Apurinic/apyrimidinic endonuclease 2 Apurinic/apyrimidinic endonuclease like 2 C430040P13Rik DNA (apurinic or apyrimidinic site) lyase 2 XTH 2 XTH2 APEX2_HUMAN. |
| Product description | Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. |
| Immunogen | KLH conjugated synthetic peptide derived from human APEX2 301-200/518 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | AP endonuclease 2 AP endonuclease XTH2 APE 2 APE2 APEX 2 APEX L2 APEX nuclease (apurinic/apyrimidinic endonuclease) 2 APEX Nuclease 2 APEX nuclease like 2 APEXL 2 APEXL2 Apurinic apyrimidinic endonuclease 2 Apurinic/apyrimidinic endonuclease 2 Apurinic/apyrimidinic endonuclease like 2 C430040P13Rik DNA (apurinic or apyrimidinic site) lyase 2 XTH 2 XTH2 APEX2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Mitochondrion (Probable). Note=Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. |
| SwissProt ID | Q9UBZ4 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-APEX2 Polyclonal Antibody, Unconjugated(ER1903-76) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Lymph node(Mouse) Lysate at 40 ug Primary: Anti- APEX2 (ER1903-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 57kD
Application
Fig3: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- APEX2 (ER1903-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 57kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (APEX2) Polyclonal Antibody, Unconjugated (ER1903-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (APEX2) Polyclonal Antibody, Unconjugated (ER1903-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Highly expressed in brain and kidney. Weakly expressed in the fetal brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
