Application
Fig1: Sample: Blood(Mouse) Cell Lysate at 30 ug Primary: Anti- APOB48R (ER1903-79) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kDApplication
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- APOB48R (ER1903-79) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD| Product Name | APOB48R |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | apo b-100 APOB apoB-48R apoB-100R apoB100R Apolipoprotein B-100 receptor Apolipoprotein B100 receptor Apolipoprotein B48 receptor. |
| Product description | Macrophage receptor that binds to the apolipoprotein B48 (APOB) of dietary triglyceride (TG)-rich lipoproteins (TRL) or to a like domain of APOB in hypertriglyceridemic very low density lipoprotein (HTG-VLDL). Binds and internalizes TRL when out of the context of the macrophage. May provide essential lipids to reticuloendothelial cells. Could also be involved in foam cell formation with elevated TRL and remnant lipoprotein (RLP). Mediates the rapid high-affinity uptake of chylomicrons (CM), HTG-VLDL, and trypsinized (tryp) VLDL devoid of APOE in vitro in macrophages. |
| Immunogen | KLH conjugated synthetic peptide derived from human APOB48R 1001-1088/1088 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogRabbit |
| Concentration | 1mg/ml |
| Alternative Names | apo b-100 APOB apoB-48R apoB-100R apoB100R Apolipoprotein B-100 receptor Apolipoprotein B100 receptor Apolipoprotein B48 receptor. |
|---|---|
| Molecular Weight(MW) | 115 KDa |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Note=Binds monocyte-macrophage membrane. Thought to be anchored in the membrane through an interaction with an integral membrane protein. |
| SwissProt ID | Q0VD83 |
|---|
Application
Fig1: Sample: Blood(Mouse) Cell Lysate at 30 ug Primary: Anti- APOB48R (ER1903-79) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- APOB48R (ER1903-79) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD| Positive Control | Expressed in peripheral blood leukocytes > bone marrow = spleen > lymph node, and only faintly visible in appendix and thymus. Expressed in the brain, heart, kidney, liver, lung, pancreas, and placenta. Expressed primarily by reticuloendothelial cells: monocytes, macrophages, and endothelial cells. Expressed in atherosclerotic lesion foam cells. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
