Application
Fig1: Western blot analysis of EGR2 on MCF-7 and SH-SY-5Y cell lysates using anti-EGR2 at 1/500 dilution.Application
Fig2: Flow cytometric analysis of SH-SY-5Y cells with EGR2 antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | EGR2 [JG78-39] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AT591 antibody CMT1D antibody CMT4E antibody DKFZp686J1957 antibody E3 SUMO-protein ligase EGR2 antibody Early growth response 2 antibody Early growth response protein 2 antibody EGR-2 antibody egr2 antibody EGR2_HUMAN antibody FLJ14547 antibody KROX 20 Drosophila homolog antibody Krox 20 homolog Drosophila antibody KROX-20, Drosophila, homolog (early growth response-2) antibody KROX20 antibody Krox20 protein antibody Zinc finger protein Krox-20 antibody |
| Product description | The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Binds to two specific DNA sites located in the promoter region of HOXA4. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen | Recombinant protein within human EGR2 aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AT591 antibody
CMT1D antibody
CMT4E antibody
DKFZp686J1957 antibody
E3 SUMO-protein ligase EGR2 antibody
Early growth response 2 antibody
Early growth response protein 2 antibody
EGR-2 antibody
egr2 antibody
EGR2_HUMAN antibody
FLJ14547 antibody
KROX 20 Drosophila homolog antibody
Krox 20 homolog Drosophila antibody
KROX-20 Drosophila homolog (early growth response-2) antibody KROX20 antibody Krox20 protein antibody Zinc finger protein Krox-20 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P11161 |
|---|
Application
Fig1: Western blot analysis of EGR2 on MCF-7 and SH-SY-5Y cell lysates using anti-EGR2 at 1/500 dilution.
Application
Fig2: Flow cytometric analysis of SH-SY-5Y cells with EGR2 antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, SH-SY-5Y, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
