Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-APPBP1 (ER1903-83) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Product Name | APPBP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 59 kDa A 116A10.1 Amyloid beta precursor protein binding protein 1 59kD Amyloid beta precursor protein binding protein 1 59kDa Amyloid beta precursor protein binding protein 1 Amyloid beta precursor protein-binding protein 1 Amyloid protein binding protein 1 Amyloid protein-binding protein 1 APP BP1 APP-BP1 APPBP 1 HPP 1 HPP1 NAE 1 NAE1 NEDD8 activating enzyme E1 regulatory subunit NEDD8 activating enzyme E1 subunit 1 NEDD8 activating enzyme E1 subunit NEDD8-activating enzyme E1 regulatory subunit Proto oncogene protein 1 Proto-oncogene protein 1 Protooncogene protein 1 ula 1 ULA1_HUMAN. |
| Product description | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. |
| Immunogen | KLH conjugated synthetic peptide derived from human APPBP1 401-500/534 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 59 kDa A 116A10.1 Amyloid beta precursor protein binding protein 1 59kD Amyloid beta precursor protein binding protein 1 59kDa Amyloid beta precursor protein binding protein 1 Amyloid beta precursor protein-binding protein 1 Amyloid protein binding protein 1 Amyloid protein-binding protein 1 APP BP1 APP-BP1 APPBP 1 HPP 1 HPP1 NAE 1 NAE1 NEDD8 activating enzyme E1 regulatory subunit NEDD8 activating enzyme E1 subunit 1 NEDD8 activating enzyme E1 subunit NEDD8-activating enzyme E1 regulatory subunit Proto oncogene protein 1 Proto-oncogene protein 1 Protooncogene protein 1 ula 1 ULA1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 60 KDa |
| Cellular Localization | Cell membrane. Colocalizes with APP in lipid rafts. |
| SwissProt ID | Q13564 |
|---|
Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-APPBP1 (ER1903-83) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Positive Control | Ubiquitous in fetal tissues. Expressed throughout the adult brain. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
