Application
Fig1: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-AQP3 antibody (ER1903-84) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Lung (mouse) Lysate at 40 ug Primary: Anti- AQP3 (ER1903-84)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32kD Observed band size: 27kD| Product Name | AQP3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AQP 3 AQP3 Aquaporin 3 (GIL blood group) Aquaporin 3 (Gill blood group) Aquaporin3 Aquaporin-3 GIL Gill blood group AQP3_MOUSE. |
| Product description | Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse AQP3 201-292/292 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AQP 3
AQP3
Aquaporin 3 (GIL blood group) Aquaporin 3 (Gill blood group) Aquaporin3 Aquaporin-3 GIL Gill blood group AQP3_MOUSE. |
|---|---|
| Molecular Weight(MW) | 32 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | Q8R2N1 |
|---|
Application
Fig1: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-AQP3 antibody (ER1903-84) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Lung (mouse) Lysate at 40 ug Primary: Anti- AQP3 (ER1903-84)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32kD Observed band size: 27kD| Positive Control | Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
