Application
Fig1: Sample:Intestinal(Mouse) lysate at 30 ug Primary: Anti-AQP9 (ER1903-85) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size : 32 kD Observed band size : 32 kDApplication
Fig2: Sample:Liver(Mouse) lysate at 30 ug Primary: Anti-AQP9 (ER1903-85) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 32 kD Observed band size : 32 kDApplication
Fig3: Blank control: A549(blue). Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibody (ER1903-85 /AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1903-85/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-AQP9 (ER1903-85) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD| Product Name | AQP9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | aquaporin Protein-9 Aquaporin 9 AQP 9 AQP9 Aquaporin9 HsT 17287 HsT17287 Small solute channel 1 SSC 1 SSC1 Aquaporin-9 AQP-9 Aquaglyceroporin-9 AQP9_HUMAN. |
| Product description | Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human AQP9 201-295/295 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | aquaporin Protein-9 Aquaporin 9 AQP 9 AQP9 Aquaporin9 HsT 17287 HsT17287 Small solute channel 1 SSC 1 SSC1 Aquaporin-9 AQP-9 Aquaglyceroporin-9 AQP9_HUMAN. |
|---|---|
| Molecular Weight(MW) | 32 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | O43315 |
|---|
Application
Fig1: Sample:Intestinal(Mouse) lysate at 30 ug Primary: Anti-AQP9 (ER1903-85) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size : 32 kD Observed band size : 32 kD
Application
Fig2: Sample:Liver(Mouse) lysate at 30 ug Primary: Anti-AQP9 (ER1903-85) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 32 kD Observed band size : 32 kD
Application
Fig3: Blank control: A549(blue). Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibody (ER1903-85 /AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1903-85/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-AQP9 (ER1903-85) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD| Positive Control | Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
