Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Aquaporin 2 (ER1903-86) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kDApplication
Fig2: Sample: Kidney (Mouse) Lysate at 40 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- Aquaporin 2 (ER1903-86) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 32 kD| Product Name | Aquaporin 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH water channel AQP 2 AQP-2 AQP CD AQP2 AQPCD Aquaporin 2 collecting duct Aquaporin CD Aquaporin2 Aquaporine 2 Collecting duct water channel protein MGC34501 Water channel protein for renal collecting duct WCH CD WCHCD. |
| Product description | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. |
| Immunogen | KLH conjugated synthetic peptide derived from human AQP2 101-200/271 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADH water channel AQP 2 AQP-2 AQP CD AQP2 AQPCD Aquaporin 2 collecting duct Aquaporin CD Aquaporin2 Aquaporine 2 Collecting duct water channel protein MGC34501 Water channel protein for renal collecting duct WCH CD WCHCD. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Apical cell membrane. Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane. |
| SwissProt ID | P41181 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Aquaporin 2 (ER1903-86) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kD
Application
Fig2: Sample: Kidney (Mouse) Lysate at 40 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- Aquaporin 2 (ER1903-86) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 32 kD| Positive Control | Expressed in renal collecting tubules. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
