Application
Fig1: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(ER1903-87) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(ER1903-87) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(ER1903-87) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Sample: mouse lung lysate at 30ug; Primary: Anti-Aquaporin 4 (ER1903-87) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bse-0295G-HRP) at 1: 5000; Predicted band size:36 kD Observed band size:34 kDApplication
Fig5: Sample: Muscle(Mouse) lysate at 30ug; Primary: Anti-Aquaporin 4 (ER1903-87) at 1:300; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:36kD Observed band size:35kDApplication
Fig6: Blank control: K562 (blue). Primary Antibody:Rabbit Anti-Aquaporin 4 antibody (ER1903-87,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (ER1903-87, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | Aquaporin 4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | aquaporin 4 WCH4 Mercurial-insensitive water channel MIWC AQP 4 AQP4 Aquaporin4 HMIWC 2 HMIWC2 Mercurial insensitive water channel MGC22454 MIWC AQP4_HUMAN Aquaporin-4 AQP-4. |
| Product description | Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. |
| Immunogen | KLH conjugated synthetic peptide derived from human AQP4 271-323/323 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | aquaporin 4 WCH4 Mercurial-insensitive water channel MIWC AQP 4 AQP4 Aquaporin4 HMIWC 2 HMIWC2 Mercurial insensitive water channel MGC22454 MIWC AQP4_HUMAN Aquaporin-4 AQP-4. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | P55088 |
|---|
Application
Fig1: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(ER1903-87) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(ER1903-87) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(ER1903-87) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Sample: mouse lung lysate at 30ug; Primary: Anti-Aquaporin 4 (ER1903-87) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bse-0295G-HRP) at 1: 5000; Predicted band size:36 kD Observed band size:34 kD
Application
Fig5: Sample: Muscle(Mouse) lysate at 30ug; Primary: Anti-Aquaporin 4 (ER1903-87) at 1:300; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:36kD Observed band size:35kD
Application
Fig6: Blank control: K562 (blue). Primary Antibody:Rabbit Anti-Aquaporin 4 antibody (ER1903-87,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (ER1903-87, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Brain, muscle, heart, kidney, lung, and trachea. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
