Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- Aquaporine 2 (ER1903-88) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 32 kDApplication
Fig2: Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ER1903-88) at 1:250 followed by conjugation to the secondary antibody and DAB stainingApplication
Fig3: Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ER1903-88) at 1:250 followed by conjugation to the secondary antibody and DAB stainingApplication
Fig4: Sample: Kidney (Rat) Lysate at 40 ug Primary: Anti-Aquaporine 2 (ER1903-88) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Product Name | Aquaporine 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH water channel AQP 2 AQP CD AQP2 AQPCD Aquaporin 2 collecting duct Aquaporin CD Aquaporin2 Aquaporine 2 Collecting duct water channel protein MGC34501 Water channel protein for renal collecting duct WCH CD WCHCD AQP2_HUMAN. |
| Product description | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. |
| Immunogen | KLH conjugated synthetic peptide derived from human AQP2. 171-271/271 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | ADH water channel AQP 2 AQP CD AQP2 AQPCD Aquaporin 2 collecting duct Aquaporin CD Aquaporin2 Aquaporine 2 Collecting duct water channel protein MGC34501 Water channel protein for renal collecting duct WCH CD WCHCD AQP2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. |
| SwissProt ID | P41181 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- Aquaporine 2 (ER1903-88) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 32 kD
Application
Fig2: Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ER1903-88) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Application
Fig3: Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ER1903-88) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Application
Fig4: Sample: Kidney (Rat) Lysate at 40 ug Primary: Anti-Aquaporine 2 (ER1903-88) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Positive Control | Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
