Application
Fig1: Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.Application
Fig2: ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.| Product Name | Alcohol Dehydrogenase [JG82-31] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH alpha subunit antibody ADH antibody ADH1 antibody ADH1A antibody ADH1A_HUMAN antibody Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody Alcohol dehydrogenase 1 antibody Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody Alcohol dehydrogenase 1A antibody Alcohol dehydrogenase subunit alpha antibody Aldehyde reductase antibody |
| Product description | The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster. The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract. |
| Immunogen | Recombinant protein within human Alcohol Dehydrogenase aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCICCIF |
| WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADH alpha subunit antibody
ADH antibody
ADH1 antibody
ADH1A antibody
ADH1A_HUMAN antibody
Alcohol dehydrogenase 1 (class I) alpha polypeptide antibody Alcohol dehydrogenase 1 antibody Alcohol dehydrogenase 1A (class I) alpha polypeptide antibody Alcohol dehydrogenase 1A antibody Alcohol dehydrogenase subunit alpha antibody Aldehyde reductase antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.| Positive Control | Rat liver tissue, human liver tissue, mouse liver tissue, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
