Application
Fig1: Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution. Positive control: Lane 1: 293 Lane 2: MCF-7 Lane 3: SK-Br-3 Lane 4: Mouse kidneyApplication
Fig2: ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | STUB1 [JG38-22] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen NY CO 7 antibody Antigen NY-CO-7 antibody C terminus of Hsp70-interacting protein antibody Carboxy terminus of Hsp70 interacting protein antibody Carboxy terminus of Hsp70-interacting protein antibody Carboxy terminus of Hsp70p interacting protein antibody CHIP antibody CHIP_HUMAN antibody CLL associated antigen KW 8 antibody CLL-associated antigen KW-8 antibody E3 ubiquitin protein ligase CHIP antibody E3 ubiquitin-protein ligase CHIP antibody Heat shock protein A binding protein 2 (c terminal) antibody HSPABP2 antibody NY CO 7 antibody PP1131 antibody SDCCAG7 antibody Serologically defined colon cancer antigen 7 antibody STIP1 homology and U Box containing protein 1 antibody STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody STIP1 homology and U box-containing protein 1 antibody STUB 1 antibody STUB1 antibody UBOX 1 antibody UBOX1 antibody |
| Product description | CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain. |
| Immunogen | Recombinant protein within human STUB1 aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Antigen NY CO 7 antibody Antigen NY-CO-7 antibody C terminus of Hsp70-interacting protein antibody Carboxy terminus of Hsp70 interacting protein antibody Carboxy terminus of Hsp70-interacting protein antibody Carboxy terminus of Hsp70p interacting protein antibody CHIP antibody CHIP_HUMAN antibody CLL associated antigen KW 8 antibody CLL-associated antigen KW-8 antibody E3 ubiquitin protein ligase CHIP antibody E3 ubiquitin-protein ligase CHIP antibody Heat shock protein A binding protein 2 (c terminal) antibody HSPABP2 antibody NY CO 7 antibody PP1131 antibody SDCCAG7 antibody Serologically defined colon cancer antigen 7 antibody STIP1 homology and U Box containing protein 1 antibody STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody STIP1 homology and U box-containing protein 1 antibody STUB 1 antibody STUB1 antibody UBOX 1 antibody UBOX1 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution. Positive control: Lane 1: 293 Lane 2: MCF-7 Lane 3: SK-Br-3 Lane 4: Mouse kidney
Application
Fig2: ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | 293, MCF-7, SK-Br-3, mouse kidney tissue, 293T, LOVO, rat epididymis tissue, human colon cancer tissue, human placenta tissue, SH-SY-5Y. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
