Application
Fig1: Sample: MDA-MB-231 (human)cell Lysate at 40 ug Primary: Anti- ASAP1 (ER1903-96)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125kD Observed band size: 125 kD| Product Name | ASAP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMAP 1 AMAP1 AMAP-1 ARF GTPase activating protein 1 ASAP 1 Centaurin beta 4 CentB4 DDEF 1 DDEF1 protein PAG 2 PAG2 PAG-2 PAP PIP2 dependent ARF1 GAP ZG 14P ZG14P Development and differentiation enhancing factor 1 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein ADP ribosylation factor directed GTPase activating protein 1 ASAP1_HUMAN. |
| Product description | Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human ASAP1 (154-190aa) 154-190/1129 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | AMAP 1 AMAP1 AMAP-1 ARF GTPase activating protein 1 ASAP 1 Centaurin beta 4 CentB4 DDEF 1 DDEF1 protein PAG 2 PAG2 PAG-2 PAP PIP2 dependent ARF1 GAP ZG 14P ZG14P Development and differentiation enhancing factor 1 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein ADP ribosylation factor directed GTPase activating protein 1 ASAP1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 125 KDa |
| Cellular Localization | Cytoplasm. Membrane. Note=Predominantly cytoplasmic. Partially membrane-associated |
| SwissProt ID | Q9ULH1 |
|---|
Application
Fig1: Sample: MDA-MB-231 (human)cell Lysate at 40 ug Primary: Anti- ASAP1 (ER1903-96)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125kD Observed band size: 125 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
