Application
Fig1: Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated(ER1904-02) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: NIH/3T3(mouse) Cell Lysate at 40 ug Primary: Anti-ATPaseNa＋/K＋ beta2 (ER1904-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig3: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated(ER1904-02) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-ATPase Na+/ K+ beta 2 (ER1904-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Product Name | ATPase Na+/ K+ beta 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | adhesion molecule on glia Na+K+ATPase AMOG AT1B2 AT1B2_HUMAN ATP1B2 ATPase Na+/K+ transporting beta 2 polypeptide ATPB2 ATPB2S MGC93648 Na+/K+ -ATPase beta 2 subunit Na, K ATPase beta 2 polypeptide RATATPB2S sodium potassium ATPase subunit beta 2 (non-catalytic) sodium pump subunit beta 2 sodium/potassium dependent ATPase beta 2 subunit Sodium/potassium dependent ATPase subunit beta 2 sodium/potassium transporting ATPase beta 2 chain sodium/potassium transporting ATPase subunit beta 2 Sodium/potassium-dependent ATPase subunit beta-2 Sodium/potassium-transporting ATPase subunit beta-2. |
| Product description | This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. |
| Immunogen | KLH conjugated synthetic peptide derived from human ATP1b2 201-290/290 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | adhesion molecule on glia
Na+K+ATPase
AMOG
AT1B2
AT1B2_HUMAN
ATP1B2
ATPase Na+/K+ transporting beta 2 polypeptide
ATPB2
ATPB2S
MGC93648
Na+/K+ -ATPase beta 2 subunit
Na K ATPase beta 2 polypeptide RATATPB2S sodium potassium ATPase subunit beta 2 (non-catalytic) sodium pump subunit beta 2 sodium/potassium dependent ATPase beta 2 subunit Sodium/potassium dependent ATPase subunit beta 2 sodium/potassium transporting ATPase beta 2 chain sodium/potassium transporting ATPase subunit beta 2 Sodium/potassium-dependent ATPase subunit beta-2 Sodium/potassium-transporting ATPase subunit beta-2. |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Membrane; Single-pass type II membrane protein. |
| SwissProt ID | P14415 |
|---|
Application
Fig1: Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated(ER1904-02) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: NIH/3T3(mouse) Cell Lysate at 40 ug Primary: Anti-ATPaseNa＋/K＋ beta2 (ER1904-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig3: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated(ER1904-02) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-ATPase Na+/ K+ beta 2 (ER1904-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
