Application
Fig1: Sample: 293T (Human)Cell Lysate at 40 ug Primary: Anti-Aurora C(ER1904-04)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 36kD Observed band size:35 kDApplication
Fig2: Sample: Cerebrum(Mouse)Lysate at 40 ug Primary: Anti-Aurora C(ER1904-04)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 36kD Observed band size: 36kD| Product Name | Aurora C |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IPL-1/STK13/Aurora C AURKC AIE2 AIK3 AurC AURKC Aurora C Aurora/Ipl1 related kinase 3 IPL-1 Aurora/Ipl1/Eg2 protein 2 EC 2.7.11.1 Serine threonine protein kinase 13 STK13 AURKC_HUMAN. |
| Product description | Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'. |
| Immunogen | KLH conjugated synthetic peptide derived from human Aurora C 51-150/344 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | IPL-1/STK13/Aurora C AURKC AIE2 AIK3 AurC AURKC Aurora C Aurora/Ipl1 related kinase 3 IPL-1 Aurora/Ipl1/Eg2 protein 2 EC 2.7.11.1 Serine threonine protein kinase 13 STK13 AURKC_HUMAN. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Note=Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remains in the midbody during cytokinesis. |
| SwissProt ID | Q9UQB9 |
|---|
Application
Fig1: Sample: 293T (Human)Cell Lysate at 40 ug Primary: Anti-Aurora C(ER1904-04)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 36kD Observed band size:35 kD
Application
Fig2: Sample: Cerebrum(Mouse)Lysate at 40 ug Primary: Anti-Aurora C(ER1904-04)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 36kD Observed band size: 36kD| Positive Control | Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cell lines such as Hep-G2, Huh-7 and HeLa. Expression is maximum at M phase. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
