Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (B4GALNT1) Polyclonal Antibody, Unconjugated (ER1904-07) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Stomach (Mouse) Lysate at 40 ug Primary: Anti- B4GALNT1/GM2 synthase (ER1904-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kDApplication
Fig3: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- B4GALNT1/GM2 synthase (ER1904-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 53 kD| Product Name | B4GALNT1/GM2 synthase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | (N acetylneuraminyl) galactosylglucosylceramide (N-acetylneuraminyl)-galactosylglucosylceramide 4 N-acetylgalactosaminyltransferase 1 4933429D13Rik B4GALNT1 B4GN1_HUMAN Beta 1 4 N acetyl galactosaminyl transferase 1 Beta 1 4 N acetylgalactosaminyltransferase 1 Beta-1 BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE 1 BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE Beta1 4GalNAc T Gal-NAc-T GALGT Galgt1 GalNAc T GalNAc-T GALNACT GD2 synthase Ggm-2 Ggm2 GM2 synthase GM2/GD2 synthase SIAT 2 SIAT2 UDP Gal:betaGlcNAc beta 1 4 N acetylgalactosaminyltransferase transferase 1 UDP N acetyl alpha D galactosamine:(N acetylneuraminyl). |
| Product description | Involved in the biosynthesis of gangliosides GM2, GD2 and GA2. |
| Immunogen | KLH conjugated synthetic peptide derived from human B4GALNT1/GM2 synthase 451-533/533 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | (N acetylneuraminyl) galactosylglucosylceramide
(N-acetylneuraminyl)-galactosylglucosylceramide
4 N-acetylgalactosaminyltransferase 1
4933429D13Rik
B4GALNT1
B4GN1_HUMAN
Beta 1 4 N acetyl galactosaminyl transferase 1
Beta 1 4 N acetylgalactosaminyltransferase 1
Beta-1
BETA-1 4-N-ACETYLGALACTOSAMINYLTRANSFERASE 1 BETA-1 4-N-ACETYLGALACTOSAMINYLTRANSFERASE Beta1 4GalNAc T Gal-NAc-T GALGT Galgt1 GalNAc T GalNAc-T GALNACT GD2 synthase Ggm-2 Ggm2 GM2 synthase GM2/GD2 synthase SIAT 2 SIAT2 UDP Gal:betaGlcNAc beta 1 4 N acetylgalactosaminyltransferase transferase 1 UDP N acetyl alpha D galactosamine:(N acetylneuraminyl). |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein. |
| SwissProt ID | Q00973 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (B4GALNT1) Polyclonal Antibody, Unconjugated (ER1904-07) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Stomach (Mouse) Lysate at 40 ug Primary: Anti- B4GALNT1/GM2 synthase (ER1904-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Application
Fig3: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- B4GALNT1/GM2 synthase (ER1904-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 53 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
