Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CD80 (ER1904-08) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 60 kDApplication
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-CD80 (ER1904-08) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 60 kDApplication
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug SP2/0 (Mouse) CellLysate at 30 ug Primary: Anti-B7-1/CD80 (ER1904-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 60 kD| Product Name | B7-1/CD80 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activation B7-1 antigen B lymphocyte activation antigen B7 B7 B7-1 B7-1 antigen BB1 CD28 antigen ligand 1 CD28LG CD28LG1 CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) CD80 antigen CD80 molecule CD80_MOUSE Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80. |
| Product description | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse B7-1/CD80 1-100/306 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Alternative Names | Activation B7-1 antigen
B lymphocyte activation antigen B7
B7
B7-1
B7-1 antigen
BB1
CD28 antigen ligand 1
CD28LG
CD28LG1
CD80
CD80 antigen (CD28 antigen ligand 1 B7-1 antigen) CD80 antigen CD80 molecule CD80_MOUSE Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80. |
|---|---|
| Molecular Weight(MW) | 32 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q00609 |
|---|
Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CD80 (ER1904-08) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 60 kD
Application
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-CD80 (ER1904-08) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 60 kD
Application
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug SP2/0 (Mouse) CellLysate at 30 ug Primary: Anti-B7-1/CD80 (ER1904-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 60 kD| Positive Control | Expressed on activated B-cells, macrophages and dendritic cells. |
|---|---|
| Application Notes | WB:1:500-1000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
