Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (B7-H6) Polyclonal Antibody, Unconjugated (ER1904-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Huh7 Cell (Human) Lysate at 40 ug A549 Cell (Human) Lysate at 40 ug Primary: Anti-B7-H6 (ER1904-09) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 48 kD Observed band size: 48 kDApplication
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti- B7-H6 (ER1904-09) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kDApplication
Fig4: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- B7-H6 (ER1904-09) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD| Product Name | B7-H6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B7 homolog 6 B7-H6 B7H6 B7H6_HUMAN DKFZp686O24166 Natural cytotoxicity triggering receptor 3 ligand 1 NCR3LG1 Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167. |
| Product description | Triggers NCR3-dependent natural killer cell activation. |
| Immunogen | KLH conjugated synthetic peptide derived from human B7-H6 51-150/454 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | B7 homolog 6 B7-H6 B7H6 B7H6_HUMAN DKFZp686O24166 Natural cytotoxicity triggering receptor 3 ligand 1 NCR3LG1 Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
| SwissProt ID | Q68D85 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (B7-H6) Polyclonal Antibody, Unconjugated (ER1904-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Huh7 Cell (Human) Lysate at 40 ug A549 Cell (Human) Lysate at 40 ug Primary: Anti-B7-H6 (ER1904-09) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti- B7-H6 (ER1904-09) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD
Application
Fig4: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- B7-H6 (ER1904-09) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD| Positive Control | Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
