Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-BAMBI (ER1904-11) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kDApplication
Fig2: Sample: RAW264.7 (Mouse) CellLysate at 30 ug Primary: Anti-BAMBI (ER1904-11) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD| Product Name | BAMBI |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BAMBI BAMBI_HUMAN BMP and activin membrane bound inhibitor homolog BMP and activin membrane-bound inhibitor homolog NMA Non metastatic gene A protein Non-metastatic gene A protein Putative transmembrane protein NMA. |
| Product description | This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human BAMBI 101-200/260 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | BAMBI
BAMBI_HUMAN
BMP and activin membrane bound inhibitor homolog BMP and activin membrane-bound inhibitor homolog NMA Non metastatic gene A protein Non-metastatic gene A protein Putative transmembrane protein NMA. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q13145 |
|---|
Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-BAMBI (ER1904-11) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD
Application
Fig2: Sample: RAW264.7 (Mouse) CellLysate at 30 ug Primary: Anti-BAMBI (ER1904-11) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD| Positive Control | High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
