Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (ER1904-12) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (ER1904-12) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-BarX2 (ER1904-12) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD| Product Name | BarX2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BarH like homeobox 2 BarX 2 BARX homeobox BARX homeobox 2 Barx2 BARX2_HUMAN Homeobox protein BarH like 2 Homeobox protein BarH-like 2 MGC133368 MGC133369. |
| Product description | Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia. |
| Immunogen | KLH conjugated synthetic peptide derived from human BarX2 81-180/279 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | BarH like homeobox 2 BarX 2 BARX homeobox BARX homeobox 2 Barx2 BARX2_HUMAN Homeobox protein BarH like 2 Homeobox protein BarH-like 2 MGC133368 MGC133369. |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9UMQ3 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (ER1904-12) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (ER1904-12) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-BarX2 (ER1904-12) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD| Positive Control | Highly expressed in adult salivary gland and at much lower levels in mammary gland, kidney and placenta. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
