Application
Fig1: Western blot analysis of Ube2N on different lysates using anti-Ube2N antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: SH-SY-5Y Lane 3: Mouse spleen Lane 4: Rat spleen| Product Name | Ube2N [JG38-68] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bendless like ubiquitin conjugating enzyme antibody Bendless-like ubiquitin-conjugating enzyme antibody BLU antibody EC 6.3.2.19 antibody Epididymis secretory protein Li 71 antibody HEL-S-71 antibody Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody MGC131857 antibody MGC8489 antibody UBC 13 antibody Ubc13 antibody UbCH ben antibody UbcH-ben antibody UbcH13 antibody UBCHBEN antibody Ube 2N antibody Ube2n antibody UBE2N_HUMAN antibody Ubiquitin carrier protein N antibody Ubiquitin conjugating enzyme E2 N antibody Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody Ubiquitin conjugating enzyme E2N antibody Ubiquitin protein ligase N antibody Ubiquitin-conjugating enzyme E2 N antibody Ubiquitin-protein ligase N antibody Yeast UBC13 homolog antibody |
| Product description | Ube2N, a member of the E2 Ubiquitin-conjugating enzyme family, completes the second step of ubiquitination reactions, essentially targeting proteins for proteasomic degradation. When proteins are modified with ubiquitin in an important cellular mechanism targeting abnormal or short lived proteins, ubiquitin, a short protein of 76 amino acids, attaches to a lysine residue resting on the target protein. Multiple cycles of ubiquitination create a polyubiquitin chain that the proteasome recognizes and subsequently triggers the ATP-dependent unfolding of the target protein. This allows protoeolytic degradation of the target protein. These degradation products are highly expressed in heart and skeletal muscles. Ube2N moderates the transcriptional activation of target genes, affects the progress of cell differentiation and aging, and also influences the DNA repair pathway, further adding to the survival of cells after DNA damage. |
| Immunogen | Recombinant protein within human Ube2N aa 1-150. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Bendless like ubiquitin conjugating enzyme antibody Bendless-like ubiquitin-conjugating enzyme antibody BLU antibody EC 6.3.2.19 antibody Epididymis secretory protein Li 71 antibody HEL-S-71 antibody Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody MGC131857 antibody MGC8489 antibody UBC 13 antibody Ubc13 antibody UbCH ben antibody UbcH-ben antibody UbcH13 antibody UBCHBEN antibody Ube 2N antibody Ube2n antibody UBE2N_HUMAN antibody Ubiquitin carrier protein N antibody Ubiquitin conjugating enzyme E2 N antibody Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody Ubiquitin conjugating enzyme E2N antibody Ubiquitin protein ligase N antibody Ubiquitin-conjugating enzyme E2 N antibody Ubiquitin-protein ligase N antibody Yeast UBC13 homolog antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of Ube2N on different lysates using anti-Ube2N antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: SH-SY-5Y Lane 3: Mouse spleen Lane 4: Rat spleen| Positive Control | Daudi, SH-SY-5Y, mouse spleen tissue lysate, rat spleen tissue lysate, human colon tissue, human prostate cancer tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
