Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated(ER1904-14) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Blank control: U-87MG(blue). Primary Antibody:Rabbit Anti-BBC3 PUMA antibody(ER1904-14), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1904-14,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig3: Sample: K562 Cell Lysate at 30 ug Primary: Anti- BBC3 (ER1904-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 25 kD| Product Name | BBC3/PUMA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PUMA bcl-2 binding component 3 BBC 3 BBC3 BCL 2 binding component 3 BCL2 binding component 3 JFY 1 JFY1 p53 up regulated modulator of apoptosis p53 Upregulated Modulator of Apoptosis PUMA alpha PUMA/JFY1 BBC3_RAT. |
| Product description | Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Essential for ER stress-induced cell death. |
| Immunogen | KLH conjugated synthetic peptide derived from human BBC3 131-180/193 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatDogPigCow |
| Concentration | 1mg/ml |
| Alternative Names | PUMA bcl-2 binding component 3 BBC 3 BBC3 BCL 2 binding component 3 BCL2 binding component 3 JFY 1 JFY1 p53 up regulated modulator of apoptosis p53 Upregulated Modulator of Apoptosis PUMA alpha PUMA/JFY1 BBC3_RAT. |
|---|---|
| Molecular Weight(MW) | 21 KDa |
| Cellular Localization | Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release. |
| SwissProt ID | Q96PG8 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated(ER1904-14) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Blank control: U-87MG(blue). Primary Antibody:Rabbit Anti-BBC3 PUMA antibody(ER1904-14), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1904-14,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: K562 Cell Lysate at 30 ug Primary: Anti- BBC3 (ER1904-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 25 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
