Application
Fig1: Sample: Adrenal gland (Mouse) Lysate at 40 ug Primary: Anti-BBS4 (ER1904-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kDApplication
Fig2: Sample: TM4 Cell (Mouse) Lysate at 40 ug Primary: Anti-BBS4 (ER1904-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Product Name | BBS4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bardet Biedl syndrome 4 protein Bardet-Biedl syndrome 4 protein Bbs4 BBS4_HUMAN. |
| Product description | May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. |
| Immunogen | KLH conjugated synthetic peptide derived from human BBS4 431-519/519 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Bardet Biedl syndrome 4 protein Bardet-Biedl syndrome 4 protein Bbs4 BBS4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton. Cell projection, cilium membrane. Cytoplasm. Note=Localizes to the pericentriolar region throughout the cell cycle. Centrosomal localization requires dynein. Localizes to nonmembranous centriolar satellites in the cytoplasm. |
| SwissProt ID | Q96RK4 |
|---|
Application
Fig1: Sample: Adrenal gland (Mouse) Lysate at 40 ug Primary: Anti-BBS4 (ER1904-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD
Application
Fig2: Sample: TM4 Cell (Mouse) Lysate at 40 ug Primary: Anti-BBS4 (ER1904-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Positive Control | Ubiquitously expressed. The highest level of expression is found in the kidney. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
