Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-BCAT1 (ER1904-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 45 kDApplication
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BCAT1 Polyclonal Antibody, Unconjugated(ER1904-16) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-BCAT1 (ER1904-16) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 43 kD Observed band size: 43 kD| Product Name | BCAT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cytosolic BCAT BCAT(c) Bcat1 BCAT1_HUMAN BCT1 Branched chain amino acid aminotransferase, cytosolic Branched-chain-amino-acid aminotransferase ECA39 MECA39 PNAS 121 Protein ECA39. |
| Product description | Branched chain amino acid transaminase 1 is a protein that in humans is encoded by the BCAT1 gene. It is the first enzyme in the Branched-chain amino acid (BCAA) degradation pathway and facilitates the reversible transamination of BCAAs and glutamate. BCAT1 resides in the cytoplasm, while its isoform, BCAT2, is found in the mitochondria. This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids (BCKAs) to the branched-chain amino acids (BCAAs) Valine, Leucine and Isoleucine, which are essential for cell growth. In humans, its primary role is the deamination of BCAAs, as humans lack the enzymes for de novo synthesis of BCKAs. The respective cofactors are alpha-ketoglutarate and glutamate. Cells can further degrade BCKAs by the Branched-chain keto acid dehydrogenase complex from which the carbon backbones of each BCAA may enter distinct degradation pathways. The oncogenic transcription factor Myc is frequently reported to drive BCAT1 expression. |
| Immunogen | KLH conjugated synthetic peptide derived from human BCAT1 321-386/386 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | cytosolic
BCAT
BCAT(c) Bcat1 BCAT1_HUMAN BCT1 Branched chain amino acid aminotransferase cytosolic Branched-chain-amino-acid aminotransferase ECA39 MECA39 PNAS 121 Protein ECA39. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P54687 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-BCAT1 (ER1904-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 45 kD
Application
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BCAT1 Polyclonal Antibody, Unconjugated(ER1904-16) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-BCAT1 (ER1904-16) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 43 kD Observed band size: 43 kD| Positive Control | During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
