Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Bcl 2 like 13 protein (ER1904-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kDApplication
Fig2: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-Bcl 2 like 13 protein (ER1904-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kD| Product Name | Bcl 2 like 13 protein |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis facilitator Bcl 2 like 13 Bcl 2 like 13 protein BCL2 like 13 apoptosis facilitator BCL2 like 13 Bcl2 like 13 protein BCL2L13 MIL 1 MIL1 MIL1 protein Protein Mil1 B2L13_HUMAN. |
| Product description | BCL2-like 13 (apoptosis facilitator), also known as BCL2L13 or Bcl-rambo, is a protein which in humans is encoded by the BCL2L13 gene on chromosome 22. This gene encodes a mitochondrially-localized protein which is classified under the Bcl-2 protein family. Overexpression of the encoded protein results in apoptosis. As a result, it has been implicated in cancers such as childhood acute lymphoblastic leukemia (ALL) and glioblastoma multiforme (GBM). Alternatively spliced transcript variants have been observed for this gene, such as Bcl-rambo beta. Bcl-rambo is a member of the Bcl-2 family of proteins that regulate apoptosis. In cells, Bcl-rambo is localized to the mitochondria, and its overexpression induces apoptosis that is blocked by caspase inhibitors, whereas inhibitors controlling upstream events of either the 'death receptor' (FLIP, FADD-DN) or the 'mitochondrial' pro-apoptotic pathway (Bcl-x(L)) had no effect. Bcl-rambo mediates apoptosis by associating with adenine nucleotide translocator (ANT), a component of the mitochondrial permeability transition pore, to induce its opening. ANT will also facilitate the transfer of ADP and ATP between the cytosol and the matrix. |
| Immunogen | KLH conjugated synthetic peptide derived from human Bcl rambo/Bcl 2 like 13 protein 281-380/485 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseChickenDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis facilitator
Bcl 2 like 13
Bcl 2 like 13 protein BCL2 like 13 apoptosis facilitator BCL2 like 13 Bcl2 like 13 protein BCL2L13 MIL 1 MIL1 MIL1 protein Protein Mil1 B2L13_HUMAN. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Isoform 2: Mitochondrion membrane; Single-pass membrane protein (Potential). Nucleus. Isoform 1: Nucleus |
| SwissProt ID | Q9BXK5 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Bcl 2 like 13 protein (ER1904-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kD
Application
Fig2: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-Bcl 2 like 13 protein (ER1904-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kD| Positive Control | Ubiquitous, with the highest levels of expression in heart, placenta and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
