Application
Fig1: Western blot analysis of TNPO3 on K562 cell (1), HL-60 cell (2) and mouse pancreas tissue (3) lysate using anti-TNPO3 antibody at 1/1,000 dilution.Application
Fig2: ICC staining TNPO3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining TNPO3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining TNPO3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.| Product Name | TNPO3 [JG38-77] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNPO 3 antibody Imp12 antibody Importin 12 antibody Importin-12 antibody IPO12 antibody MTR10A antibody TNPO3 antibody TNPO3_HUMAN antibody Transportin 3 antibody Transportin SR antibody Transportin-3 antibody Transportin-SR antibody TRN SR antibody TRN-SR antibody TRN-SR2 antibody TRNSR antibody |
| Product description | The Importin complex consists of Importin-α and Importin-β proteins which assist in transport of arginine- or serine-rich (SR) proteins across the nucleus. Importin-12, also known as Transportin-3 or Transportin-SR, is a member of the Importin-β family and functions as a nuclear transport receptor for serine/arginine-rich proteins. Through recognition of phosphorylated RS domains, Importin-12 mediates the nuclear import of several SR proteins, such as splicing factors SFRS1 and SFRS2. By regulating the nucleocytoplasmic transport of these and other SR mRNA splicing factors, Importin-12 controls their access to mRNA and, therefore, acts a transcriptional regulator. |
| Immunogen | Recombinant protein within human TNPO3 aa 800-950. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | TNPO 3 antibody Imp12 antibody Importin 12 antibody Importin-12 antibody IPO12 antibody MTR10A antibody TNPO3 antibody TNPO3_HUMAN antibody Transportin 3 antibody Transportin SR antibody Transportin-3 antibody Transportin-SR antibody TRN SR antibody TRN-SR antibody TRN-SR2 antibody TRNSR antibody |
|---|---|
| Molecular Weight(MW) | 104 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of TNPO3 on K562 cell (1), HL-60 cell (2) and mouse pancreas tissue (3) lysate using anti-TNPO3 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining TNPO3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TNPO3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining TNPO3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.| Positive Control | HL-60, mouse pancreas tissue lysate, A549, SH-SY-5Y, SiHa, human liver cancer tissue, human small intestine tissue, mouse lung tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
