Application
Fig1: Sample: Lung(Mouse) lysate at 30ug; Primary: Anti-beta Glucan Receptor (ER1904-20) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution; Predicted band size: 28 kD Observed band size: 28 kDApplication
Fig2: Sample: Lung (Rat) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-beta Glucan Receptor (ER1904-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 38 kDApplication
Fig3: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-beta Glucan Receptor (ER1904-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28/38 kD| Product Name | beta Glucan Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dectin1 Dectin-1 Dectin 1 CLECSF12 C-type lectin domain family 7 member A beta Glucan Receptor Beta GR BGR C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 Clec7a CANDF4 DECTIN1 CLEC7A protein Clecsf12 Dectin 1 DECTIN 1 receptor Dectin1 Dendritic cell associated C type lectin 1 Lectin like receptor 1 beta Glucan Receptor Putative transmembrane protein dectin 1 CLC7A_HUMAN. |
| Product description | Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human Dectin-1 41-140/247 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Dectin1 Dectin-1 Dectin 1 CLECSF12 C-type lectin domain family 7 member A beta Glucan Receptor Beta GR BGR C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 Clec7a CANDF4 DECTIN1 CLEC7A protein Clecsf12 Dectin 1 DECTIN 1 receptor Dectin1 Dendritic cell associated C type lectin 1 Lectin like receptor 1 beta Glucan Receptor Putative transmembrane protein dectin 1 CLC7A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. |
| SwissProt ID | Q9BXN2 |
|---|
Application
Fig1: Sample: Lung(Mouse) lysate at 30ug; Primary: Anti-beta Glucan Receptor (ER1904-20) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution; Predicted band size: 28 kD Observed band size: 28 kD
Application
Fig2: Sample: Lung (Rat) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-beta Glucan Receptor (ER1904-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 38 kD
Application
Fig3: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-beta Glucan Receptor (ER1904-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28/38 kD| Positive Control | Detected in dendritic cells, in paracortical and medullary regions of lymph nodes, and in spleen red pulp and white pulp. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
