Application
Fig1: Sample: MCF-7 Cell (Human) Lysate at 40 ug Primary: Anti-BIN2 (ER1904-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 63 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BIN2) Polyclonal Antibody, Unconjugated (ER1904-24) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | BIN2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BIN2 BIN2_HUMAN BRAP 1 Breast cancer associated protein BRAP1 Breast cancer-associated protein 1 Bridging integrator 2. |
| Immunogen | KLH conjugated synthetic peptide derived from human BIN2/BRAP1 21-120/565 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | BIN2 BIN2_HUMAN BRAP 1 Breast cancer associated protein BRAP1 Breast cancer-associated protein 1 Bridging integrator 2. |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9UBW5 |
|---|
Application
Fig1: Sample: MCF-7 Cell (Human) Lysate at 40 ug Primary: Anti-BIN2 (ER1904-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 63 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BIN2) Polyclonal Antibody, Unconjugated (ER1904-24) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
